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Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.

Anti-NTF4 Antibody

HPA049376
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
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Product group Antibodies
TargetNTF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NTF4
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4909
  • Target name
    NTF4
  • Target description
    neurotrophin 4
  • Target synonyms
    GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; neurotrophin-5; neutrophic factor 4; NT4; NT-4; NT-4/5; NT5; NT-5; NTF5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP34130
  • Protein Name
    Neurotrophin-4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NT-4/NTF4 using anti-NT-4/NTF4 antibody (A06935-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT-4/NTF4 antigen affinity purified polyclonal antibody (Catalog # A06935-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT-4/NTF4 at approximately 24KD. The expected band size for NT-4/NTF4 is at 24KD.
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