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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Anti-NUBPL Antibody

HPA029203
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUBPL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUBPL
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80224
  • Target name
    NUBPL
  • Target description
    NUBP iron-sulfur cluster assembly factor, mitochondrial
  • Target synonyms
    C14orf127, IND1, MC1DN21, huInd1, iron-sulfur cluster transfer protein NUBPL, IND1 homolog, iron-sulfur protein required for NADH dehydrogenase, nucleotide binding protein like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TB37
  • Protein Name
    Iron-sulfur protein NUBPL
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NUBPL using anti-NUBPL antibody (A10634-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Colo320 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBPL antigen affinity purified polyclonal antibody (Catalog # A10634-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUBPL at approximately 34 kDa. The expected band size for NUBPL is at 34 kDa.
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