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Western blot All lanes: NUBPL antibody at 1microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 19 kDa Observed band size: 35 kDa
Western blot All lanes: NUBPL antibody at 1microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 19 kDa Observed band size: 35 kDa
Western blot All lanes: NUBPL antibody at 1microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 19 kDa Observed band size: 35 kDa

NUBPL Antibody

CSB-PA819450LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNUBPL
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Overview

  • Supplier
    Cusabio
  • Product Name
    NUBPL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80224
  • Target name
    NUBPL
  • Target description
    nucleotide binding protein like
  • Target synonyms
    C14orf127; huInd1; IND1; IND1 homolog; iron-sulfur protein NUBPL; iron-sulfur protein required for NADH dehydrogenase; MC1DN21
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TB37
  • Protein Name
    Iron-sulfur protein NUBPL
  • Scientific Description
    Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver of one or more Fe-S clusters to complex I subunits.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUBPL using anti-NUBPL antibody (A10634-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Colo320 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBPL antigen affinity purified polyclonal antibody (Catalog # A10634-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUBPL at approximately 34 kDa. The expected band size for NUBPL is at 34 kDa.
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