Bio-Connect

Nucleotide binding protein like antibody

GTX131851
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNUBPL
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    Nucleotide binding protein like antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80224
  • Target name
    NUBPL
  • Target description
    nucleotide binding protein like
  • Target synonyms
    C14orf127; huInd1; IND1; IND1 homolog; iron-sulfur protein NUBPL; iron-sulfur protein required for NADH dehydrogenase; MC1DN21
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TB37
  • Protein Name
    Iron-sulfur protein NUBPL
  • Scientific Description
    This gene encodes a member of the Mrp/NBP35 ATP-binding proteins family. The encoded protein is required for the assembly of the respiratory chain NADH dehydrogenase (complex I), an oligomeric enzymatic complex located in the inner mitochondrial membrane. Mutations in this gene cause mitochondrial complex I deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Western blot All lanes: NUBPL antibody at 1microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 19 kDa Observed band size: 35 kDa
Product group Antibodies
Cusabio
NUBPL AntibodyCSB-PA819450LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetNUBPL
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetNUBPL
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetNUBPL
  • SizePrice
Figure 1. Western blot analysis of NUBPL using anti-NUBPL antibody (A10634-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Colo320 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBPL antigen affinity purified polyclonal antibody (Catalog # A10634-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUBPL at approximately 34 kDa. The expected band size for NUBPL is at 34 kDa.
Product group Antibodies
Boster Bio
Anti-NUBPL Antibody Picoband(r)A10634-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetNUBPL
  • SizePrice
Hela cell lysates probed with NUBPL Polyclonal Antibody, Unconjugated (bs-19505R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNUBPL
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
NUBPL AntibodyLS-C378192
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetNUBPL
  • SizePrice
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetNUBPL
  • SizePrice
Product group Antibodies
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetNUBPL
  • SizePrice