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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Anti-NUDT15 Antibody

HPA038969
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUDT15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUDT15
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55270
  • Target name
    NUDT15
  • Target description
    nudix hydrolase 15
  • Target synonyms
    MTH2, NUDT15D, nucleotide triphosphate diphosphatase NUDT15, 8-oxo-dGTPase NUDT15, mutT homolog 2, nucleoside diphosphate-linked to another moiety X hydrolase 15, nudix (nucleoside diphosphate linked moiety X)-type motif 15, probable 7,8-dihydro-8-oxoguanine triphosphatase NUDT15, probable 8-oxo-dGTP diphosphatase NUDT15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NV35
  • Protein Name
    Nucleotide triphosphate diphosphatase NUDT15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA885733LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of NUDT15 using anti-NUDT15 antibody (A05498-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT15 antigen affinity purified polyclonal antibody (Catalog # A05498-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT15 at approximately 19 kDa. The expected band size for NUDT15 is at 19 kDa.
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