Anti-Occludin [37-5]

Absolute Antibody

Anti-Occludin [37-5]

9

This antibody is specific for the first extracellular loop (EL1) of OCLN.

The cell binding capability of the original format of this antibody (rat IgG2b) was assessed using FC, and its binding to HT1080/hOCLN cells was assessed using a cell-based ELISA. This antibody was found to have no negative effect on tight junction (TJ) barrier function, as determined by a TJ barrier function assay (TER). Furthermore, this antibody was found to have no negative effect on cell viability, as determined by a colorimetric XTT assay. This antibody was found to strongly inhibit (in vitro) and fully block (in vivo) HCV infections, as measured by ICC (Shimizu et al., 2018; PMID: 29437969). This antibodys Kd for hOCLN is 0.720 +/- 0.084 nM, as measured in a cell-based competition ELISA. This antibody successfully precipitated FLAG-hOCLN proteins found in HT1080 cell lysates and endogenously expressed cellular OCLN found in Caco-2, HepG2, and MCF-7 cell lysates. Furthermore, other TJ proteins expressed in T84 cells - TRIC and ZO-2 - were found to be co-immunoprecipitated with OCLN by this antibody. This antibody can be used for immunoblot analysis of OCLN (only at high concentrations due to a weak response). Immunofluorescence (IF) staining of OCLN using clone 1-3 with a TJ marker protein ZO-1 in Caco-2 cells was co-localized with ZO-1 at cell-cell contact sites. This antibody cross-reacts with crab-eating monkey OCLN (moOCLN), as determined by FC (Shimizu et al., 2019; PMID: 31077306).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

37-5

OCLN

occludin

BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1

Human

IgG1

Occludin

Human, Monkey

-20°C,2°C to 8°C

12352203