Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4°C, DAB staining.
Occludin Polyclonal Antibody
BS-10011R
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
TargetOCLN
Overview
- SupplierBioss
- Product NameOccludin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, Western Blot
- Applications SupplierWB(1:300-5000), FCM(1:20-100)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID100506658
- Target nameOCLN
- Target descriptionoccludin
- Target synonymsBLCPMG, PPP1R115, PTORCH1, occludin, phosphatase 1, regulatory subunit 115
- HostRabbit
- IsotypeIgG
- Protein IDQ16625
- Protein NameOccludin
- ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Qu XY, Zhang YM, Tao LN, et al. XingNaoJing injections protect against cerebral ischemia/reperfusion injury and alleviate blood-brain barrier disruption in rats, through an underlying mechanism of NLRP3 inflammasomes suppression. Chin J Nat Med. 2019,17(7):498-505. doi: 10.1016/S1875-5364(19)30071-8Read this paper
- Li X, Wang H, Zhang Y, et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019,7:e6715. doi: 10.7717/peerj.6715Read this paper
- Chen M , Liu Y , Xiong S , et al. Dietary l-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model. Food Funct. 2019,10(5):2390-2398. doi: 10.1039/c9fo00123aRead this paper
- Zhao B, Che D, Adams S, et al. N-Acetyl-d-galactosamine prevents soya bean agglutinin-induced intestinal barrier dysfunction in intestinal porcine epithelial cells. J Anim Physiol Anim Nutr (Berl). 2019,103(4):1198-1206. doi: 10.1111/jpn.13091Read this paper
- Han L, Li T, Du M, et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice. Nutrients. 2019,11(3). doi: 10.3390/nu11030670Read this paper
- Chang L, Lu Z, Li D, et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets. Toxicol Lett. 2018,296:114-124. doi: 10.1016/j.toxlet.2018.07.019Read this paper
- Xu T, Dong Z, Wang X, et al. IL-1β induces increased tight junction permeability in bovine mammary epithelial cells via the IL-1β-ERK1/2-MLCK axis upon blood-milk barrier damage. J Cell Biochem. 2018,119(11):9028-9041. doi: 10.1002/jcb.27160Read this paper
Datasheet
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