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Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Occludin Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4°C, DAB staining.

Occludin Polyclonal Antibody

BS-10011R
Bioss
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetOCLN
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Overview

  • Supplier
    Bioss
  • Product Name
    Occludin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID100506658
  • Target name
    OCLN
  • Target description
    occludin
  • Target synonyms
    BLCPMG, PPP1R115, PTORCH1, occludin, phosphatase 1, regulatory subunit 115
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16625
  • Protein Name
    Occludin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Qu XY, Zhang YM, Tao LN, et al. XingNaoJing injections protect against cerebral ischemia/reperfusion injury and alleviate blood-brain barrier disruption in rats, through an underlying mechanism of NLRP3 inflammasomes suppression. Chin J Nat Med. 2019,17(7):498-505. doi: 10.1016/S1875-5364(19)30071-8
    Read this paper
  • Li X, Wang H, Zhang Y, et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019,7:e6715. doi: 10.7717/peerj.6715
    Read this paper
  • Chen M , Liu Y , Xiong S , et al. Dietary l-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model. Food Funct. 2019,10(5):2390-2398. doi: 10.1039/c9fo00123a
    Read this paper
  • Zhao B, Che D, Adams S, et al. N-Acetyl-d-galactosamine prevents soya bean agglutinin-induced intestinal barrier dysfunction in intestinal porcine epithelial cells. J Anim Physiol Anim Nutr (Berl). 2019,103(4):1198-1206. doi: 10.1111/jpn.13091
    Read this paper
  • Han L, Li T, Du M, et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice. Nutrients. 2019,11(3). doi: 10.3390/nu11030670
    Read this paper
  • Chang L, Lu Z, Li D, et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets. Toxicol Lett. 2018,296:114-124. doi: 10.1016/j.toxlet.2018.07.019
    Read this paper
  • Xu T, Dong Z, Wang X, et al. IL-1β induces increased tight junction permeability in bovine mammary epithelial cells via the IL-1β-ERK1/2-MLCK axis upon blood-milk barrier damage. J Cell Biochem. 2018,119(11):9028-9041. doi: 10.1002/jcb.27160
    Read this paper

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