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Occludin antibody

GTX114949
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetOCLN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Occludin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.25 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID100506658
  • Target name
    OCLN
  • Target description
    occludin
  • Target synonyms
    BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16625
  • Protein Name
    Occludin
  • Scientific Description
    This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction. Chou HC et al., 2022 Jun 15, J Biomed Sci
    Read more
  • S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage. Fan X et al., 2022, Front Pharmacol
    Read more
  • Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus. Muranyi W et al., 2022 Jun 17, iScience
    Read more
  • A novel mechanism linking ferroptosis and endoplasmic reticulum stress via the circPtpn14/miR-351-5p/5-LOX signaling in melatonin-mediated treatment of traumatic brain injury. Wu C et al., 2022 Jan, Free Radic Biol Med
    Read more
  • Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes. Watanabe D et al., 2021 Sep 16, Pharmaceutics
    Read more
  • CircLphn3 protects the blood-brain barrier in traumatic brain injury. Cheng YQ et al., 2022 Apr, Neural Regen Res
    Read more
  • Proneurogenic and neuroprotective effect of a multi strain probiotic mixture in a mouse model of acute inflammation: Involvement of the gut-brain axis. Petrella C et al., 2021 Oct, Pharmacol Res
    Read more
  • Beneficial effects of Dendrobium officinale on metabolic hypertensive rats by triggering the enteric-origin SCFA-GPCR43/41 pathway. Li B et al., 2021 Jun 21, Food Funct
    Read more
  • Brain metastases-derived extracellular vesicles induce binding and aggregation of low-density lipoprotein. Busatto S et al., 2020 Nov 7, J Nanobiotechnology
    Read more
  • Guiqi Baizhu Decoction Alleviates Radiation Inflammation in Rats by Modulating the Composition of the Gut Microbiota. Zhang LY et al., 2020, Evid Based Complement Alternat Med
    Read more

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Figure 1. Western blot analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/OCLN antigen affinity purified polyclonal antibody (Catalog # A01246-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/OCLN at approximately 65KD. The expected band size for Occludin/OCLN is at 59KD.
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IHC-P analysis of mouse brain tissue using GTX85016 Occludin antibody. Red : Primary antibody Blue : DAPI Dilution : 20 microg/ml
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