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Anti-OGT Antibody

144-01990
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetOGT
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-OGT Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8473
  • Target name
    OGT
  • Target description
    O-linked N-acetylglucosamine (GlcNAc) transferase
  • Target synonyms
    HINCUT-1; HRNT1; MRX106; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT1; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase; XLID106
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15294
  • Protein Name
    UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
  • Scientific Description
    OGT Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OGT using anti-OGT antibody (PB9767). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGT antigen affinity purified polyclonal antibody (Catalog # PB9767) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGT at approximately 117KD. The expected band size for OGT is at 117KD.
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