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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic as well as weaker nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic as well as weaker nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic as well as weaker nuclear positivity in neurons.

Anti-OLA1 Antibody

HPA035790
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetOLA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OLA1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29789
  • Target name
    OLA1
  • Target description
    Obg like ATPase 1
  • Target synonyms
    DOC45, GBP45, GTBP9, GTPBP9, PTD004, obg-like ATPase 1, DNA damage-regulated overexpressed in cancer 45 protein, GTP-binding protein 9 (putative), GTP-binding protein PTD004, homologous yeast-44.2 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTK5
  • Protein Name
    Obg-like ATPase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GTPBP9/OLA1 using anti-GTPBP9/OLA1 antibody (A07162). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTPBP9/OLA1 antigen affinity purified polyclonal antibody (Catalog # A07162) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GTPBP9/OLA1 at approximately 45 kDa. The expected band size for GTPBP9/OLA1 is at 45 kDa.
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