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Western blot All lanes: OLA1 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 28, 32 kDa Observed band size: 45 kDa
Western blot All lanes: OLA1 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 28, 32 kDa Observed band size: 45 kDa
Western blot All lanes: OLA1 antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 28, 32 kDa Observed band size: 45 kDa

OLA1 Antibody

CSB-PA016318HA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetOLA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    OLA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29789
  • Target name
    OLA1
  • Target description
    Obg like ATPase 1
  • Target synonyms
    DNA damage-regulated overexpressed in cancer 45 protein; DOC45; GBP45; GTBP9; GTP-binding protein 9 (putative); GTP-binding protein PTD004; GTPBP9; homologous yeast-44.2 protein; obg-like ATPase 1; PTD004
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTK5
  • Protein Name
    Obg-like ATPase 1
  • Scientific Description
    Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GTPBP9/OLA1 using anti-GTPBP9/OLA1 antibody (A07162). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTPBP9/OLA1 antigen affinity purified polyclonal antibody (Catalog # A07162) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GTPBP9/OLA1 at approximately 45 kDa. The expected band size for GTPBP9/OLA1 is at 45 kDa.
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