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Anti-Omentin [3G1]

AB01379-1.1-BT
Absolute Antibody
ApplicationsWestern Blot
Product group Antibodies
TargetITLN1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Omentin [3G1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of this antibody for both omentin 1 and omentin 2 has been confirmed in two-dimensional Western blot analysis of purified omentin samples (de Souza Batista et al, 2007). This antibody has also been used in a quantitative Western blotting analysis of the relative abundance of omentin 1/2 in human plasma samples (de Souza Batista et al, 2007), and Western blot analysis of the culture medium of human adipose tissue explants (Yang et al, 2006).
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G1
  • Gene ID55600
  • Target name
    ITLN1
  • Target description
    intelectin 1
  • Target synonyms
    HL-1, HL1, INTL, ITLN, LFR, hIntL, omentin, intelectin-1, ITLN-1, endothelial lectin HL-1, galactofuranose-binding lectin, intelectin 1 (galactofuranose binding), intestinal lactoferrin receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8WWA0
  • Protein Name
    Intelectin-1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ITLN1 using anti-ITLN1 antibody (PB10004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITLN1 antigen affinity purified polyclonal antibody (Catalog # PB10004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITLN1 at approximately 43 kDa. The expected band size for ITLN1 is at 35 kDa.
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