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Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal intestine tissue, Primary antibody: CSB-PA522045(ITLN1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal intestine tissue, Primary antibody: CSB-PA522045(ITLN1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal intestine tissue, Primary antibody: CSB-PA522045(ITLN1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes

ITLN1 Antibody

CSB-PA522045
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetITLN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITLN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55600
  • Target name
    ITLN1
  • Target description
    intelectin 1
  • Target synonyms
    HL-1, HL1, INTL, ITLN, LFR, hIntL, omentin, intelectin-1, ITLN-1, endothelial lectin HL-1, galactofuranose-binding lectin, intelectin 1 (galactofuranose binding), intestinal lactoferrin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WWA0
  • Protein Name
    Intelectin-1
  • Scientific Description
    Intelectin-1 also known as the intestinal lactoferrin receptor is a protein that in humans is encoded by the ITLN1 gene.Intelectin-1 functions both as a receptor for bacterial arabinogalactans and for lactoferrin. Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ITLN1 using anti-ITLN1 antibody (PB10004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITLN1 antigen affinity purified polyclonal antibody (Catalog # PB10004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITLN1 at approximately 43 kDa. The expected band size for ITLN1 is at 35 kDa.
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