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Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.

Anti-OS9 Antibody

HPA013694
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetOS9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OS9
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10956
  • Target name
    OS9
  • Target description
    OS9 endoplasmic reticulum lectin
  • Target synonyms
    amplified in osteosarcoma 9; endoplasmic reticulum lectin 2; ERLEC2; erlectin 2; OS-9; osteosarcoma amplified 9, endoplasmic reticulum associated protein; osteosarcoma amplified 9, endoplasmic reticulum lectin; protein OS-9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13438
  • Protein Name
    Protein OS-9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, NIH/3T3 cells, Primary antibody: CSB-PA577148(OS9 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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Figure 1. Western blot analysis of OS9 using anti-OS9 antibody (A04158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OS9 antigen affinity purified polyclonal antibody (Catalog # A04158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OS9 at approximately 83 kDa. The expected band size for OS9 is at 76 kDa.
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