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ProductsBack/OS9 Antibody
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, NIH/3T3 cells, Primary antibody: CSB-PA577148(OS9 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, NIH/3T3 cells, Primary antibody: CSB-PA577148(OS9 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, NIH/3T3 cells, Primary antibody: CSB-PA577148(OS9 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes

OS9 Antibody

CSB-PA577148
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetOS9
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Overview

  • Supplier
    Cusabio
  • Product Name
    OS9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10956
  • Target name
    OS9
  • Target description
    OS9 endoplasmic reticulum lectin
  • Target synonyms
    ERLEC2, OS-9, protein OS-9, amplified in osteosarcoma 9, endoplasmic reticulum lectin 2, erlectin 2, osteosarcoma amplified 9, endoplasmic reticulum associated protein, osteosarcoma amplified 9, endoplasmic reticulum lectin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13438
  • Protein Name
    Protein OS-9
  • Scientific Description
    This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OS9 using anti-OS9 antibody (A04158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OS9 antigen affinity purified polyclonal antibody (Catalog # A04158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OS9 at approximately 83 kDa. The expected band size for OS9 is at 76 kDa.
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