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Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9918). Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Osteocalcin Antibody (PB9918) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9918). Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Osteocalcin Antibody (PB9918) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9918). Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Osteocalcin Antibody (PB9918) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Anti-Osteocalcin/BGLAP Antibody

PB9918-CARRIER-FREE
Boster Bio
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetBGLAP
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Osteocalcin/BGLAP Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID632
  • Target name
    BGLAP
  • Target description
    bone gamma-carboxyglutamate protein
  • Target synonyms
    BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); gamma-carboxyglutamic acid-containing protein; OC; OCN; osteocalcin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02818
  • Protein Name
    Osteocalcin
  • Scientific Description
    Boster Bio Anti-Osteocalcin/BGLAP Antibody Picoband® catalog # PB9918. Tested in IHC applications. This antibody reacts with Human.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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