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PAX5 Antibody (YA2597, Rabbit)

HY-P82852
MedChem Express
TargetPAX5
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PAX5 Antibody (YA2597, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5079
  • Target name
    PAX5
  • Target description
    paired box 5
  • Target synonyms
    ALL3; B-cell lineage specific activator; BSAP; paired box homeotic gene 5; paired box protein Pax-5; paired domain gene 5; transcription factor PAX 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PAX5 Antibody (YA2597) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2597), targeting PAX5, with a predicted molecular weight of 42 kDa (observed band size: 45 kDa). PAX5 Antibody (YA2597) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAX5 using anti-PAX5 antibody (A00669-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX5 antigen affinity purified polyclonal antibody (Catalog # A00669-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45 kDa. The expected band size for PAX5 is at 42 kDa.
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