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Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.

Anti-PCMT1 Antibody

HPA003239
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPCMT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PCMT1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5110
  • Target name
    PCMT1
  • Target description
    protein-L-isoaspartate (D-aspartate) O-methyltransferase
  • Target synonyms
    PIMT, protein-L-isoaspartate(D-aspartate) O-methyltransferase, L-isoaspartyl protein carboxyl methyltransferase, epididymis secretory sperm binding protein, protein L-isoaspartyl/D-aspartyl methyltransferase, protein-beta-aspartate methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22061
  • Protein Name
    Protein-L-isoaspartate(D-aspartate) O-methyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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IHC image of CSB-PA017618LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of PCMT1 using anti-PCMT1 antibody (A04579-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (Catalog # A04579-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCMT1 at approximately 25 kDa. The expected band size for PCMT1 is at 25, 25-28 kDa.
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