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PCMT1 Antibody

LS-C334874
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPCMT1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PCMT1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 24kDa, while the observed MW by Western blot was 25kDa.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), IP (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 24kDa, while the observed MW by Western blot was 25kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    4.14 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5110
  • Target name
    PCMT1
  • Target description
    protein-L-isoaspartate (D-aspartate) O-methyltransferase
  • Target synonyms
    PIMT, protein-L-isoaspartate(D-aspartate) O-methyltransferase, L-isoaspartyl protein carboxyl methyltransferase, epididymis secretory sperm binding protein, protein L-isoaspartyl/D-aspartyl methyltransferase, protein-beta-aspartate methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCMT1 using anti-PCMT1 antibody (A04579-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (Catalog # A04579-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCMT1 at approximately 25 kDa. The expected band size for PCMT1 is at 25, 25-28 kDa.
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