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Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PCMT1 Polyclonal Antibody, Unconjugated(bs-6211R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PCMT1 Polyclonal Antibody, Unconjugated(bs-6211R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PCMT1 Polyclonal Antibody, Unconjugated(bs-6211R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

PCMT1 Polyclonal Antibody

BS-6211R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPCMT1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PCMT1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5110
  • Target name
    PCMT1
  • Target description
    protein-L-isoaspartate (D-aspartate) O-methyltransferase
  • Target synonyms
    epididymis secretory sperm binding protein; L-isoaspartyl protein carboxyl methyltransferase; PIMT; protein L-isoaspartyl/D-aspartyl methyltransferase; protein-beta-aspartate methyltransferase; protein-L-isoaspartate(D-aspartate) O-methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22061
  • Protein Name
    Protein-L-isoaspartate(D-aspartate) O-methyltransferase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA017618LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of PCMT1 using anti-PCMT1 antibody (A04579-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (Catalog # A04579-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCMT1 at approximately 25 kDa. The expected band size for PCMT1 is at 25, 25-28 kDa.
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