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Anti-PCSK9 Antibody

HY-P990485
MedChem Express
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Anti-PCSK9 Antibody
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Estimated Purity
    98.39
  • Scientific Description
    Anti-PCSK9 Antibody is a human antibody expressed in CHO, targeting PCSK9. Anti-PCSK9 Antibody contains a huIgG1 heavy chain and a hukappa light chain, with a predicted molecular weight (MW) of 145 kDa. The isotype control for Anti-PCSK9 Antibody can refer to Human IgG1 kappa, Isotype Control (HY-P99001).
  • Storage Instruction
    -80°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of PCSK9 using anti-PCSK9 antibody (A00085-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # A00085-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa, 80 kDa (precursor). The expected band size for PCSK9 is at 72 kDa.
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