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Anti-PCTAIRE1 (PCTK1) (N-term) Antibody

102-27194
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetCDK16
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PCTAIRE1 (PCTK1) (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB8583
  • Gene ID5127
  • Target name
    CDK16
  • Target description
    cyclin dependent kinase 16
  • Target synonyms
    cell division protein kinase 16; cyclin-dependent kinase 16; PCTAIRE; PCTAIRE1; PCTAIRE-motif protein kinase 1; PCTGAIRE; PCTK1; serine/threonine-protein kinase PCTAIRE-1; testis secretory sperm-binding protein Li 224n
  • Host
    Rabbit
  • Protein IDQ00536
  • Protein Name
    Cyclin-dependent kinase 16
  • Scientific Description
    Rabbit Anti-PCTAIRE1 (PCTK1) (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CDK16 using anti-CDK16 antibody (A06102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK16 antigen affinity purified polyclonal antibody (Catalog # A06102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK16 at approximately 56KD. The expected band size for CDK16 is at 56KD.
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