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CDK16 Antibody (YA515, Rabbit)

HY-P80612
MedChem Express
TargetCDK16
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CDK16 Antibody (YA515, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5127
  • Target name
    CDK16
  • Target description
    cyclin dependent kinase 16
  • Target synonyms
    PCTAIRE, PCTAIRE1, PCTGAIRE, PCTK1, cyclin-dependent kinase 16, PCTAIRE-motif protein kinase 1, cell division protein kinase 16, serine/threonine-protein kinase PCTAIRE-1, testis secretory sperm-binding protein Li 224n
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CDK16 Antibody (YA515) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK16.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDK16 using anti-CDK16 antibody (A06102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK16 antigen affinity purified polyclonal antibody (Catalog # A06102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK16 at approximately 56KD. The expected band size for CDK16 is at 56KD.
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