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Western blot All lanes: CDK16 antibody at 5ug/ml Lane 1: Mouse brain tissue Lane 2: Mouse small intestineSecondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 64, 57 kDa Observed band size: 56 kDa
Western blot All lanes: CDK16 antibody at 5ug/ml Lane 1: Mouse brain tissue Lane 2: Mouse small intestineSecondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 64, 57 kDa Observed band size: 56 kDa
Western blot All lanes: CDK16 antibody at 5ug/ml Lane 1: Mouse brain tissue Lane 2: Mouse small intestineSecondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 64, 57 kDa Observed band size: 56 kDa

CDK16 Antibody

CSB-PA017648ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCDK16
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDK16 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5127
  • Target name
    CDK16
  • Target description
    cyclin dependent kinase 16
  • Target synonyms
    cell division protein kinase 16; cyclin-dependent kinase 16; PCTAIRE; PCTAIRE1; PCTAIRE-motif protein kinase 1; PCTGAIRE; PCTK1; serine/threonine-protein kinase PCTAIRE-1; testis secretory sperm-binding protein Li 224n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00536
  • Protein Name
    Cyclin-dependent kinase 16
  • Scientific Description
    Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at Ser-336 (in vitro).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDK16 using anti-CDK16 antibody (A06102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK16 antigen affinity purified polyclonal antibody (Catalog # A06102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK16 at approximately 56KD. The expected band size for CDK16 is at 56KD.
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