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Immunohistochemical staining of human adrenal gland shows strong positivity in cortical cells.
Immunohistochemical staining of human adrenal gland shows strong positivity in cortical cells.
Immunohistochemical staining of human adrenal gland shows strong positivity in cortical cells.

Anti-PDCD10 Antibody

HPA027095
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDCD10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDCD10 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11235
  • Target name
    PDCD10
  • Target description
    programmed cell death 10
  • Target synonyms
    CCM3, TFAR15, programmed cell death protein 10, TF-1 cell apoptosis-related protein 15, apoptosis-related protein 15, cerebral cavernous malformations 3 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BUL8
  • Protein Name
    Programmed cell death protein 10
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Lane 1: Hela cell lysates; Lane 2: PDCD10 knockout (KO) HeLa cell lysates probed with PDCD10 Polyclonal Antibody, Unconjugated (bs-55165R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of PDCD10 using anti-PDCD10 antibody (A01879-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat smooth muscle tissue lysates, Lane 2: mouse smooth muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD10 antigen affinity purified polyclonal antibody (Catalog # A01879-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD10 at approximately 29KD. The expected band size for PDCD10 is at 25KD.
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WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35229 PDCD10 antibody. Dilution : 1:1000 Loading : 25microg per lane
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The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA469157(PDCD10 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
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