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The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA469157(PDCD10 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA469157(PDCD10 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA469157(PDCD10 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

PDCD10 Antibody

CSB-PA469157
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPDCD10
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDCD10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11235
  • Target name
    PDCD10
  • Target description
    programmed cell death 10
  • Target synonyms
    apoptosis-related protein 15; CCM3; cerebral cavernous malformations 3 protein; programmed cell death protein 10; TF-1 cell apoptosis-related protein 15; TFAR15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BUL8
  • Protein Name
    Programmed cell death protein 10
  • Scientific Description
    This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDCD10 using anti-PDCD10 antibody (A01879-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat smooth muscle tissue lysates, Lane 2: mouse smooth muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD10 antigen affinity purified polyclonal antibody (Catalog # A01879-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD10 at approximately 29KD. The expected band size for PDCD10 is at 25KD.
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