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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm, plasma membrane & cell junctions.
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm, plasma membrane & cell junctions.
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm, plasma membrane & cell junctions.

Anti-PDGFRA Antibody

HPA004947
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPDGFRA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDGFRA Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5156
  • Target name
    PDGFRA
  • Target description
    platelet derived growth factor receptor alpha
  • Target synonyms
    CD140A, PDGFR-2, PDGFR2, platelet-derived growth factor receptor alpha, CD140 antigen-like family member A, CD140a antigen, PDGF-R-alpha, alpha-type platelet-derived growth factor receptor, platelet-derived growth factor receptor 2, platelet-derived growth factor receptor, alpha polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16234
  • Protein Name
    Platelet-derived growth factor receptor alpha
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PDGFRA using anti-PDGFRA antibody (A00366). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRA antigen affinity purified polyclonal antibody (Catalog # A00366) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFRA at approximately 180 kDa. The expected band size for PDGFRA is at 123,180 kDa.
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Human tissue extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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