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Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

Anti-PDK3 Antibody

HPA046583
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDK3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDK3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5165
  • Target name
    PDK3
  • Target description
    pyruvate dehydrogenase kinase 3
  • Target synonyms
    CMTX6, GS1-358P8.4, pyruvate dehydrogenase kinase, isozyme 3, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, pyruvate dehydrogenase kinase, isoenzyme 3, pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15120
  • Protein Name
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PDK3 using anti-PDK3 antibody (A05658-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human U251 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK3 antigen affinity purified polyclonal antibody (Catalog # A05658-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK3 at approximately 47 kDa. The expected band size for PDK3 is at 47 kDa.
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