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Western blot All lanes: PDK3 antibody at 1.6microg/ml Lane 1: Mouse kidney tissue Lane 2: RAW264.7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 49 kDa Observed band size: 47 kDa
Western blot All lanes: PDK3 antibody at 1.6microg/ml Lane 1: Mouse kidney tissue Lane 2: RAW264.7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 49 kDa Observed band size: 47 kDa
Western blot All lanes: PDK3 antibody at 1.6microg/ml Lane 1: Mouse kidney tissue Lane 2: RAW264.7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 49 kDa Observed band size: 47 kDa

PDK3 Antibody

CSB-PA613585ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPDK3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDK3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5165
  • Target name
    PDK3
  • Target description
    pyruvate dehydrogenase kinase 3
  • Target synonyms
    CMTX6, GS1-358P8.4, pyruvate dehydrogenase kinase, isozyme 3, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, pyruvate dehydrogenase kinase, isoenzyme 3, pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15120
  • Protein Name
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial
  • Scientific Description
    Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDK3 using anti-PDK3 antibody (A05658-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human U251 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK3 antigen affinity purified polyclonal antibody (Catalog # A05658-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK3 at approximately 47 kDa. The expected band size for PDK3 is at 47 kDa.
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