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Western blot analysis of lysates from 293 cells, treated with Heat shock, using PFKFB2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, treated with Heat shock, using PFKFB2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, treated with Heat shock, using PFKFB2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PFKFB2/P Pfk 2 Car Antibody

A30440
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPFKFB2
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PFKFB2/P Pfk 2 Car Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID5208
  • Target name
    PFKFB2
  • Target description
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
  • Target synonyms
    PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme, 6PF-2-K/Fru-2,6-P2ase 2, PFK/FBPase 2, PFKFB, cardiac, fructose-2,6-bisphosphatase, cardiac isozyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60825
  • Protein Name
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
  • Scientific Description
    Boster Bio Anti-PFKFB2/P Pfk 2 Car Antibody (Catalog# A30440). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PFKFB2 using anti-PFKFB2 antibody (A05977). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse heart tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB2 antigen affinity purified polyclonal antibody (Catalog # A05977) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKFB2 at approximately 58KD. The expected band size for PFKFB2 is at 58KD.
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