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Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.
Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.
Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.

anti-Phospho-Smad1 (Ser463/465) / Smad5 (Ser463/465) / Smad9 (Ser465/467), Rabbit Monoclonal (RM487)

REV-31-1379-00
RevMAb Biosciences
ApplicationsWestern Blot
Product group Antibodies
TargetSMAD1
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-Phospho-Smad1 (Ser463/465) / Smad5 (Ser463/465) / Smad9 (Ser465/467), Rabbit Monoclonal (RM487)
  • Delivery Days Customer
    10
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM487
  • Gene ID4086
  • Target name
    SMAD1
  • Target description
    SMAD family member 1
  • Target synonyms
    BSP1; BSP-1; JV41; JV4-1; MAD, mothers against decapentaplegic homolog 1; MADH1; MADR1; Mad-related protein 1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15198
  • Protein Name
    Mothers against decapentaplegic homolog 9
  • Scientific Description
    Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, 8, 9; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor-beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5, 8, 9 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads. - Recombinant Antibody. This antibody reacts to Smad1 and Smad5 when phosphorylated at Ser463/465 and Smad9 (Smad8) when phosphorylated at Ser465/467. There is no cross-reactivity to Smad1, Smad5, or Smad9 that are not phosphorylated. This antibody reacts to human, mouse or rat Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467). Source: Rabbit. Isotype: Rabbit IgG. Immunogen: A phospho-peptide corresponding to human Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467). Applications: WB. Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, 8, 9; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor-beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5, 8, 9 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (A00728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse kidney tissue lysate, Lane 5: mouse testis tissue lysate, Lane 6: human Hela cell lysate, Lane 7: human A375 cell lysate, Lane 8: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (Catalog # A00728-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1 at approximately 52,60KD. The expected band size for SMAD1 is at 48, 52, 60KD.
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