Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat C6 whole cell lysate, Lane 5: mouse thymus tissue lysate, Lane 6: mouse spleen tissue lysate, Lane 7: mouse brain tissue lysate, Lane 8: mouse Neuro-2a whole cell lysate, Lane 9: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC beta 1 at approximately 77KD. The expected band size for PKC beta 1 is at 77KD.