Figure 1. Western blot analysis of PRKCB using anti-PRKCB antibody (A01940). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCB antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCB at approximately 77 kDa. The expected band size for PRKCB is at 77 kDa.
Anti-PKC beta 1/PRKCB Antibody Picoband(r)
A01940
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKCB
Overview
- SupplierBoster Bio
- Product NameAnti-PKC beta 1/PRKCB Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5579
- Target namePRKCB
- Target descriptionprotein kinase C beta
- Target synonymsPKCB; PKC-B; PKCbeta; PKC-beta; PKCI(2); PRKCB1; PRKCB2; protein kinase C beta type; protein kinase C, beta 1 polypeptide
- HostRabbit
- IsotypeIgG
- Protein IDP05771
- Protein NameProtein kinase C beta type
- Scientific DescriptionBoster Bio Anti-PKC beta 1/PRKCB Antibody Picoband® catalog # A01940. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203