Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat C6 whole cell lysate, Lane 5: mouse thymus tissue lysate, Lane 6: mouse spleen tissue lysate, Lane 7: mouse brain tissue lysate, Lane 8: mouse Neuro-2a whole cell lysate, Lane 9: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC beta 1 at approximately 77KD. The expected band size for PKC beta 1 is at 77KD.
Anti-PKC beta 1/PRKCB Antibody Picoband(r)
A01940
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKCB
Overview
- SupplierBoster Bio
- Product NameAnti-PKC beta 1/PRKCB Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5579
- Target namePRKCB
- Target descriptionprotein kinase C beta
- Target synonymsPKCB; PKC-B; PKCbeta; PKC-beta; PKCI(2); PRKCB1; PRKCB2; protein kinase C beta type; protein kinase C, beta 1 polypeptide
- HostRabbit
- IsotypeIgG
- Protein IDP05771
- Protein NameProtein kinase C beta type
- Scientific DescriptionBoster Bio Anti-PKC beta 1/PRKCB Antibody Picoband® catalog # A01940. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203