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Immunohistochemical staining of human fallopian tube shows moderate to strong positivity in plasma.
Immunohistochemical staining of human fallopian tube shows moderate to strong positivity in plasma.
Immunohistochemical staining of human fallopian tube shows moderate to strong positivity in plasma.

Anti-PLAT Antibody

HPA003412
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLAT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLAT Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5327
  • Target name
    PLAT
  • Target description
    plasminogen activator, tissue type
  • Target synonyms
    T-PA, TPA, tissue-type plasminogen activator, alteplase, plasminogen/activator kringle, reteplase, t-plasminogen activator
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00750
  • Protein Name
    Tissue-type plasminogen activator
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PLAT using anti-PLAT antibody (A02965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # A02965-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 63-69KD. The expected band size for PLAT is at 63KD.
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