
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG1 (phospho Tyr783) antibody (GTX133465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PLCG1 (phospho Tyr783) antibody
GTX133465
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPLCG1
Overview
- SupplierGeneTex
- Product NamePLCG1 (phospho Tyr783) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.27 mg/ml
- ConjugateUnconjugated
- Gene ID5335
- Target namePLCG1
- Target descriptionphospholipase C gamma 1
- Target synonymsIDAA, NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate, PLC-148, PLC-gamma-1, inositoltrisphosphohydrolase, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phosphoinositide phospholipase C, phosphoinositide phospholipase C-gamma-1, phospholipase C, gamma 1 (formerly subtype 148), phospholipase C-148, phospholipase C-II, triphosphoinositide phosphodiesterase
- HostRabbit
- IsotypeIgG
- Protein IDP19174
- Protein Name1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
- Scientific DescriptionThe protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203







![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG1 antibody [HL3508] (GTX641396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641396/GTX641396_T-45607_20241129_WB_M_24120522_853.webp)
![Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PLCG1 (phospho Ser1248) antibody [HL3719] (GTX641909) diluted at 1:1000 and PLCG1 antibody (GTX641396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641909/GTX641909_T-45663_20250124_WB_treatment_EGF_25020422_950.webp)