Anti-PLC-gamma phosphorylated [AbAb1-PLCy]

Absolute Antibody

Anti-PLC-gamma phosphorylated [AbAb1-PLCy]

9

This antibody binds phosphorylated phosphoinositide phospholipase C-gamma, which mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It also plays an important role in the regulation of in

The binding reactivity of this antibody to recombinant phosphorylated pPLC-gamma peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of phosphorylated PLC-gamma from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pPLC-gamma. The fluorescent scFv version of this antibody, when expressed intracellularly was reported to inhibit signal transduction by PLC-gamma in transfected into NIH3T3 cells as observed using fluorescent microscopy (US20090233358).

ImmunoPrecipitation, Western Blot, ELISA

Research Use Only

Monoclonal

AbAb1-PLCy

PLCG1

phospholipase C gamma 1

1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; NCKAP3; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-148; phospholipase C-II; PLC1; PLC148; PLC-148; PLCgamma1; PLC-gamma-1; PLC-II; triphosphoinositide phosphodiesterase

Human

IgG1

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG format, created for improved compatibility with existing reagents, assays and techniques.

Human, Mouse

-20°C,2°C to 8°C

12352203