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Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

Anti-POGLUT1 Antibody

HPA037856
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOGLUT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POGLUT1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56983
  • Target name
    POGLUT1
  • Target description
    protein O-glucosyltransferase 1
  • Target synonyms
    C3orf9, CLP46, KDELCL1, KTELC1, LGMD2Z, LGMDR21, MDS010, MDSRP, Rumi, hCLP46, protein O-glucosyltransferase 1, 9630046K23Rik, CAP10-like 46 kDa protein, KDELC family like 1, KTEL (Lys-Tyr-Glu-Leu) containing 1, KTEL motif-containing protein 1, O-glucosyltransferase Rumi homolog, hRumi, myelodysplastic syndromes relative protein, protein O-xylosyltransferase POGLUT1, x 010 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBL1
  • Protein Name
    Protein O-glucosyltransferase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of POGLUT1 using anti-POGLUT1 antibody (A06826-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGLUT1 antigen affinity purified polyclonal antibody (Catalog # A06826-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGLUT1 at approximately 55 kDa. The expected band size for POGLUT1 is at 55 kDa.
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