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ProductsBack/POGLUT1 Antibody
Western Blot Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate All lanes: POGLUT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate All lanes: POGLUT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate All lanes: POGLUT1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa

POGLUT1 Antibody

CSB-PA818754LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOGLUT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    POGLUT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56983
  • Target name
    POGLUT1
  • Target description
    protein O-glucosyltransferase 1
  • Target synonyms
    9630046K23Rik; C3orf9; CAP10-like 46 kDa protein; CLP46; hCLP46; hRumi; KDELC family like 1; KDELCL1; KTEL (Lys-Tyr-Glu-Leu) containing 1; KTEL motif-containing protein 1; KTELC1; LGMD2Z; LGMDR21; MDS010; MDSRP; myelodysplastic syndromes relative protein; O-glucosyltransferase Rumi homolog; protein O-glucosyltransferase 1; protein O-xylosyltransferase POGLUT1; Rumi; x 010 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBL1
  • Protein Name
    Protein O-glucosyltransferase 1
  • Scientific Description
    Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076). Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (PubMed:21081508, PubMed:21490058, PubMed:21949356, PubMed:27807076). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (PubMed:27807076). Notch glucosylation does not affect Notch ligand binding (PubMed:21490058). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POGLUT1 using anti-POGLUT1 antibody (A06826-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGLUT1 antigen affinity purified polyclonal antibody (Catalog # A06826-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGLUT1 at approximately 55 kDa. The expected band size for POGLUT1 is at 55 kDa.
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