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anti-POLL / POLkappa antibody

ARG63382
Arigo Biolaboratories
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPOLL
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-POLL / POLkappa antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: Recommend incubate at RT for 1h.* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27343
  • Target name
    POLL
  • Target description
    DNA polymerase lambda
  • Target synonyms
    BETAN, POLKAPPA, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, polymerase (DNA directed), lambda, polymerase (DNA) lambda
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UGP5
  • Protein Name
    DNA polymerase lambda
  • Scientific Description
    This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNA Polymerase lambda using anti-DNA Polymerase lambda antibody (A00959-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase lambda antigen affinity purified polyclonal antibody (Catalog # A00959-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase lambda at approximately 70KD. The expected band size for DNA Polymerase lambda is at 63KD.
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