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Anti-POLR2A Antibody

144-11181
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPOLR2A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-POLR2A Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5430
  • Target name
    POLR2A
  • Target description
    RNA polymerase II subunit A
  • Target synonyms
    DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24928
  • Protein Name
    DNA-directed RNA polymerase II subunit RPB1
  • Scientific Description
    POLR2A Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR2A using anti-POLR2A antibody (A01029-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A antigen affinity purified polyclonal antibody (Catalog # A01029-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2A at approximately 220KD. The expected band size for POLR2A is at 220KD.
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