Anti-RNA polymerase II [CTD 4H8]

Absolute Antibody

Anti-RNA polymerase II [CTD 4H8]

7

This mAb has been used in a variety of methods, including Western blotting of the unphosphorylated (Engelhardt et al, 2005) or Ser5-CTD phosphorylated form (Stock et al, 2007), and in precipitation experiments either simply pulling down RNAPII (Kukalev et al, 2005; Peng et al, 2014) or coupled to massively parallel DNA sequencing (ChIP-seq) of the total RNAPII-associated DNA isolated from human CD4+ cells (Zhang et al, 2012).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry

ELISA; FACS; IHC; IP; IF; WB; ChIP; ChIP-seq

Research Use Only

Monoclonal

CTD 4H8

POLR2A

RNA polymerase II subunit A

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Mouse

IgG1

DNA-directed RNA polymerase II subunit RPB1

Human, Saccharomyces cerevisiae

Murine antibodies were produced by immunising mice with a peptide consisting of 10 repeats of the RNA polymerase II C-terminal domain sequence YSPTSPS. In each repeat, Ser5 was phosphorylated. Immunised cells were fused with the Sp2/0-Ag14 myeloma cell line to produce a hybridoma.

-20°C,2°C to 8°C

12352203