Paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with RNA polymerase 2 Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with RNA polymerase 2 Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
RNA polymerase II Polyclonal Antibody
BS-6972R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPOLR2A
Overview
- SupplierBioss
- Product NameRNA polymerase II Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID5430
- Target namePOLR2A
- Target descriptionRNA polymerase II subunit A
- Target synonymsNEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, DNA-directed RNA polymerase II subunit RPB1, 3'-5' exoribonuclease, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, polymerase (RNA) II subunit A
- HostRabbit
- IsotypeIgG
- Protein IDP24928
- Protein NameDNA-directed RNA polymerase II subunit RPB1
- Storage Instruction-20°C
- UNSPSC12352203
References
- Dou YD, Zhao H, Huang T, et al. STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells. Sci Rep. 2016,6:26691. doi: 10.1038/srep26691Read this paper
Datasheet
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