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Paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with RNA polymerase 2 Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with RNA polymerase 2 Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with RNA polymerase 2 Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.

RNA polymerase 2 Polyclonal Antibody

BS-6972R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat
TargetPOLR2A
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    RNA polymerase 2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5430
  • Target name
    POLR2A
  • Target description
    RNA polymerase II subunit A
  • Target synonyms
    DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24928
  • Protein Name
    DNA-directed RNA polymerase II subunit RPB1
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells. Dou YD et al., 2016 Jun 8, Sci Rep
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