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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-PPID Antibody

HPA019692
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPID
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPID
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5481
  • Target name
    PPID
  • Target description
    peptidylprolyl isomerase D
  • Target synonyms
    40 kDa peptidyl-prolyl cis-trans isomerase D; cyclophilin 40; cyclophilin D; cyclophilin-related protein; CYP-40; CYPD; peptidyl-prolyl cis-trans isomerase D; PPIase D; rotamase D; testicular tissue protein Li 147
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08752
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase D
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PPID using anti-PPID antibody (A02424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPID antigen affinity purified polyclonal antibody (Catalog # A02424) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPID at approximately 38 kDa. The expected band size for PPID is at 41 kDa.
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