Bio-Connect
Figure 1. Western blot analysis of PPIL2 using anti-PPIL2 antibody (A11451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIL2 antigen affinity purified polyclonal antibody (Catalog # A11451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPIL2 at approximately 59 kDa. The expected band size for PPIL2 is at 59 kDa.
Figure 1. Western blot analysis of PPIL2 using anti-PPIL2 antibody (A11451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIL2 antigen affinity purified polyclonal antibody (Catalog # A11451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPIL2 at approximately 59 kDa. The expected band size for PPIL2 is at 59 kDa.
Figure 1. Western blot analysis of PPIL2 using anti-PPIL2 antibody (A11451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIL2 antigen affinity purified polyclonal antibody (Catalog # A11451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPIL2 at approximately 59 kDa. The expected band size for PPIL2 is at 59 kDa.

Anti-PPIL2 Antibody Picoband(r)

A11451-1-CARRIER-FREE
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetPPIL2
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PPIL2 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID23759
  • Target name
    PPIL2
  • Target description
    peptidylprolyl isomerase like 2
  • Target synonyms
    CYC4; cyclophilin, 60kDa; cyclophilin-60; cyclophilin-like protein CyP-60; CYP60; Cyp-60; hCyP-60; peptidylprolyl cis-trans isomerase; peptidyl-prolyl cis-trans isomerase-like 2; peptidylprolyl isomerase (cyclophilin)-like 2; PPIase; probable inactive peptidyl-prolyl cis-trans isomerase-like 2; RING-type E3 ubiquitin transferase isomerase-like 2; RING-type E3 ubiquitin-protein ligase PPIL2; rotamase PPIL2; U-box domain containing 7; UBOX7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13356
  • Protein Name
    RING-type E3 ubiquitin-protein ligase PPIL2
  • Scientific Description
    Boster Bio Anti-PPIL2 Antibody Picoband® catalog # A11451-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
Assay Genie
TargetPPIL2
  • SizePrice
Anti-PPIL2 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA621661ESR1HU at dilution of 1:100
Product group Antibodies
Cusabio
PPIL2 AntibodyCSB-PA621661ESR1HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPPIL2
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
TargetPPIL2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPPIL2
  • SizePrice
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPPIL2
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetPPIL2
  • SizePrice