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Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in both exocrine glandular cells and islet cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in both exocrine glandular cells and islet cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in both exocrine glandular cells and islet cells.

Anti-PPP1R15A Antibody

HPA020240
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetPPP1R15A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP1R15A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23645
  • Target name
    PPP1R15A
  • Target description
    protein phosphatase 1 regulatory subunit 15A
  • Target synonyms
    GADD34; growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; myeloid differentiation primary response protein MyD116 homolog; protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75807
  • Protein Name
    Protein phosphatase 1 regulatory subunit 15A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GADD34/PPP1R15A using anti-GADD34/PPP1R15A antibody (A02394-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human SIHA whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/PPP1R15A antigen affinity purified polyclonal antibody (Catalog # A02394-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/PPP1R15A at approximately 100 kDa. The expected band size for GADD34/PPP1R15A is at 73 kDa.
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