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ProductsBack/Goat anti-PPP1R15A / GADD34

Goat anti-PPP1R15A / GADD34

EB05034
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPPP1R15A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PPP1R15A / GADD34 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23645
  • Target name
    PPP1R15A
  • Target description
    protein phosphatase 1 regulatory subunit 15A
  • Target synonyms
    GADD34; growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; myeloid differentiation primary response protein MyD116 homolog; protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_055145.3. Purification: Antigen affinity purified. Names and symbols: PPP1R15A; GADD34; protein phosphatase 1, regulatory (inhibitor) subunit 15A; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GADD34/PPP1R15A using anti-GADD34/PPP1R15A antibody (A02394-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human SIHA whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/PPP1R15A antigen affinity purified polyclonal antibody (Catalog # A02394-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/PPP1R15A at approximately 100 kDa. The expected band size for GADD34/PPP1R15A is at 73 kDa.
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