Anti-Prion protein [D18]

Absolute Antibody

Anti-Prion protein [D18]

9

This antibody recognises a discontinuous epitope and binds to residues 132-156 of PrP.

The specificity of the antibody to recombinant SHaPrP(90-231) and with PrP 27-30 rods was confirmed by ELISA. The epitope mapping of this antibody was done using a paptide-based ELISA. The Fab fragment reacted with cell surface MoPrPc as showed by flow cytometry. The antibody efficiently immunoprecipitated SHaPrPC from transfected CHO cells (Williamson et al. 1998; PMID: 9765500). The ability of of the Fab fragment to inhibit prion propagation in a cultured mouse neuroblastoma cell line infected with PrPSc (ScN2a) was tested. The Fab fragment was found to abolish prion replication and to clear pre-exiting PrPSc, eliminating 50% of PrPSc from the cells within about 24 hours. Therefore, the antibody showed potential as a therapeutic agent. This antibody was used for detection of PrP with the surface of ScN2a cells by flow cytometry (Peretz et al., 2001; PMID: 11507642). The scFv version of the antibody was constructed and the specificity tested by ELISA. The binding affinity of the scFv fragment to PcP was measured by surface plasmon resonance (Kd= 10.1 nM). The scFv antibody gene was delivered to the brain by a recombinant adeno-associated virus, where it delayed the progression of the disease in a mouse model of TSE (Wuertzer et al., 2008; PMID: 18180775). The Fab fragment was conjugated to UltreSmall gold particles and used for immunolabelling for PrPC localization in mouse hippocampus (Mironov et al., 2003; PMID: 12904479). Immunofluorescence was performed on RK13 cells using this antibody (Spagnolli et al., 2021; PMID: 33437023).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

D18

PRNP

prion protein

alternative prion protein; AltPrP; ASCR; CD230; CD230 antigen; CJD; GSS; KURU; major prion protein; p27-30; prion-related protein; PRIP; PrP; PrP27-30; PrP33-35C; PrPc

Mouse

IgG2b

Major prion protein

Bovine, Hamster, Human, Mouse

-20°C,2°C to 8°C

12352203