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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Anti-PRKCA Antibody

HPA006563
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKCA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRKCA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5578
  • Target name
    PRKCA
  • Target description
    protein kinase C alpha
  • Target synonyms
    AAG6; aging-associated gene 6; PKCA; PKC-A; PKCalpha; PKC-alpha; PKCI+/-; PRKACA; protein kinase C alpha type
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17252
  • Protein Name
    Protein kinase C alpha type
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Product group Antibodies
References
Bioss Antibodies
Phospho-PKC alpha (Thr638) Polyclonal AntibodyBS-3333R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPRKCA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PRKCA Antibody144-00267
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetPRKCA
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-PKC-alpha Antibody, Affinity PurifiedA302-445A
ApplicationsImmunoPrecipitation
TargetPRKCA
  • SizePrice
Figure 1. Western blot analysis of PKC Alpha/PRKCA using anti-PKC Alpha/PRKCA antibody (A00743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (Catalog # A00743-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.
Product group Antibodies
Boster Bio
Anti-PKC Alpha/PRKCA Antibody Picoband(r)A00743-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKCA
  • SizePrice
Product group Antibodies
Biomatik
Prkca Polyclonal AntibodyCAC09189
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetPRKCA
  • SizePrice
Product group Antibodies
Assay Genie
PKC alpha AntibodyCAB0267
TargetPRKCA
  • SizePrice
Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody
Product group Antibodies
Cusabio
PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ AntibodyCSB-PA003800
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PRKCA / PKC-Alpha AntibodyLS-C402927
ApplicationsWestern Blot, ELISA
TargetPRKCA
  • SizePrice
Product group Antibodies
Abbexa
PKC alpha (Phospho-Tyr657) AntibodyABX012471
ApplicationsWestern Blot, ELISA
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Product group Antibodies
MedChem Express
Phospho-PKC alpha (T638) Antibody (YA163, Rabbit)HY-P80279
TargetPRKCA
  • SizePrice