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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.

Anti-PRKCB Antibody

HPA054203
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPRKCB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRKCB Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5579
  • Target name
    PRKCB
  • Target description
    protein kinase C beta
  • Target synonyms
    PKC-beta, PKCB, PKCI(2), PKCbeta, PRKCB1, PRKCB2, protein kinase C beta type, PKC-B, protein kinase C, beta 1 polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05771
  • Protein Name
    Protein kinase C beta type
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PRKCB using anti-PRKCB antibody (A01940). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCB antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCB at approximately 77 kDa. The expected band size for PRKCB is at 77 kDa.
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