Anti-Protein C [HPC-4]

Absolute Antibody

Anti-Protein C [HPC-4]

9

Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but

HPC-4 binds human protein C only in the presence of Ca2+; it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987; 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992; PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992; PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988; PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987; PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca2+-dependent manner, purification, and others (Tanaka et al., 2006; PMID: 16398653) (Bae et al., 2007; PMID: 17823308).

ELISA, Neutralisation/Blocking, Purification/Extraction/Isolation

Research Use Only

Monoclonal

HPC-4

PROC

protein C, inactivator of coagulation factors Va and VIIIa

activated protein C; anticoagulant protein C; APC; autoprothrombin IIA; blood coagulation factor XIV; PC; prepro-protein C; PROC1; Protein C-Nagoya; THPH3; THPH4; type I protein C; vitamin K-dependent protein C

Human

IgG1

Vitamin K-dependent protein C

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Primate

-20°C,2°C to 8°C

12352203