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Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.

Anti-PSAT1 Antibody

HPA042924
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPSAT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSAT1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29968
  • Target name
    PSAT1
  • Target description
    phosphoserine aminotransferase 1
  • Target synonyms
    EPIP, NLS2, PSA, PSAT, PSATD, phosphoserine aminotransferase, endometrial progesterone-induced protein, phosphohydroxythreonine aminotransferase, phosphoserine transaminase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y617
  • Protein Name
    Phosphoserine aminotransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PSAT1 using anti-PSAT1 antibody (A06277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAT1 antigen affinity purified polyclonal antibody (Catalog # A06277) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSAT1 at approximately 40 kDa. The expected band size for PSAT1 is at 37-45 kDa.
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